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p4ebp1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p4ebp1
    P4ebp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+thr37/Phospho-4E-BP1+(Thr37%2F46)+Rabbit+mAb/pmc13049662-43-0-3
    Average 96 stars, based on 2472 article reviews
    p4ebp1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: KPNA2 is overexpressed in human and mouse endometrial cancers and promotes cellular proliferation.
    Article Snippet: Endometrial cancer is the most frequently occurring malignancy of the female genital tract in Western countries.. Although in many cases surgically curable, about 30% of the tumours represent an aggressive and untreatable disease.. In an attempt to establish a reliable prognostic marker for endometrial carcinomas disregarding their histological diversity, we investigated the expression of KPNA2, a mediator of nucleocytoplasmic transport, and other cell proliferation-associated proteins and their correlation with cancer progression.

    Staining:

    Article Title: KPNA2 is overexpressed in human and mouse endometrial cancers and promotes cellular proliferation.
    Article Snippet: Endometrial cancer is the most frequently occurring malignancy of the female genital tract in Western countries.. Although in many cases surgically curable, about 30% of the tumours represent an aggressive and untreatable disease.. In an attempt to establish a reliable prognostic marker for endometrial carcinomas disregarding their histological diversity, we investigated the expression of KPNA2, a mediator of nucleocytoplasmic transport, and other cell proliferation-associated proteins and their correlation with cancer progression.

    other:

    Article Title: Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.
    Article Snippet: Mouse MMP-9 (1:1000, #2270), SNAIL (1:1000, #3879), SLUG (1:1000, #9585), caspase3 (1:1000, #9662), caspase-8 (1:1000, #4790), VEGFR2 (1:1000, #2479), phospho-Tyr1175VEGFR2 (1:800, #2478), phospho-Tyr1068-EGFR (1:1000, #2234), FGFR (1:1000, #9740), PDGFR2 (1:1000, #3169), phospho-Tyr751-PDGFR2 (1:800, #3166), TGFβ (1:2000, #3711), phospho-Tyr199/458-PI3K (1:1000, #4228), phospho-Ser473-Akt (1:1000, #4058), phosphoThr308-Akt (1:1000, #9275), phospho-Ser2448-mTOR (1:1000, #5536), PTEN (1:1000, #9552), phospho-Ser385-PTEN (1:1000, #9551), TSC1 (1:1000, #4906), TSC2 (1:1000, #4308), phosphoThr172-AMPKα (1:1000, #2535), phospho-Tyr416-c-Src (1:1000, #2101), phospho-Tyr1007/1008Jak2 (1:500, #3776), phospho-Tyr705-STAT3 (1:1000, #9131), phospho-Ser428-LKB-1 (1:1000, #3482), LKB-1 (1:1000, #3047), GSK3β (1:1000, #9832), phospho-Ser9-GSK3β (1:1000, #5558), phospho-Ser552-β-catenin (1:1000, #5651), phospho-Ser33/37-Thr41-β-catenin (1:1000, #9561), p70S6K (1:1000, #9202), phospho-Ser465/467-Ser423/425-Smad2/3 (1:1000, #8828), Smad4 (1:1000, #38454), phospho-Thr421-Ser424-p70S6K (1:1000, #9204), phospho-Ser338Raf1 (1:1000, #9427), phospho-Ser217/221-MEK (1:1000, #9154), 4EBP-1 (1:1000, #9644), and phospho-Thr37/46-4EBP1 (1:1500, #2855) were purchased from Cell signaling (Beverly, MA).

    Article Title: Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice
    Article Snippet: Mouse MMP-9 (1:1000, #2270), SNAIL (1:1000, #3879), SLUG (1:1000, #9585), caspase-3 (1:1000, #9662), caspase-8 (1:1000, #4790), VEGFR2 (1:1000, #2479), phospho-Tyr1175-VEGFR2 (1:800, #2478), phospho-Tyr1068-EGFR (1:1000, #2234), FGFR (1:1000, #9740), PDGFR2 (1:1000, #3169), phospho-Tyr751-PDGFR2 (1:800, #3166), TGFβ (1:2000, #3711), phospho-Tyr199/458-PI3K (1:1000, #4228), phospho-Ser473-Akt (1:1000, #4058), phospho-Thr308-Akt (1:1000, #9275), phospho-Ser2448-mTOR (1:1000, #5536), PTEN (1:1000, #9552), phospho-Ser385-PTEN (1:1000, #9551), TSC1 (1:1000, #4906), TSC2 (1:1000, #4308), phospho-Thr172-AMPKα (1:1000, #2535), phospho-Tyr416-c-Src (1:1000, #2101), phospho-Tyr1007/1008-Jak2 (1:500, #3776), phospho-Tyr705-STAT3 (1:1000, #9131), phospho-Ser428-LKB-1 (1:1000, #3482), LKB-1 (1:1000, #3047), GSK3β (1:1000, #9832), phospho-Ser9-GSK3β (1:1000, #5558), phospho-Ser552-β-catenin (1:1000, #5651), phospho-Ser33/37-Thr41-β-catenin (1:1000, #9561), p70S6K (1:1000, #9202), phospho-Ser465/467-Ser423/425-Smad2/3 (1:1000, #8828), Smad4 (1:1000, #38454), phospho-Thr421-Ser424-p70S6K (1:1000, #9204), phospho-Ser338-Raf1 (1:1000, #9427), phospho-Ser217/221-MEK (1:1000, #9154), 4EBP-1 (1:1000, #9644), and phospho-Thr37/46-4EBP1 (1:1500, #2855) were purchased from Cell signaling (Beverly, MA).

    Immunohistochemistry:

    Article Title: Mutations of the MAPK/TSC/mTOR pathway characterize periventricular glioblastoma with epithelioid SEGA-like morphology–morphological and therapeutic implications
    Article Snippet: The automated Ki-67 proliferation index was performed as previously described, by using the Nikon NIS Elements 4.51.00 program set up with an object count algorithm for recognition of the differentially labeled nuclei [ ]. .. Immunohistochemistry with antibodies for phospho-Thr37/46-4E-BP1 (236B4) and total 4E-BP1 (53H11) (Cell Signaling Technology, Danvers, MA, USA) at 1:1000 and 1:2000 dilution, respectively, was manually performed as described [ ]. ..

    Article Title: Combined Vhl , Trp53 and Rb1 mutation causes clear cell renal cell carcinoma in mice
    Article Snippet: .. Immunohistochemistry and immunofluorescence were conducted using previously described methods using the antibodies against the following antigens AQP1 (Abcam, ab15080), AQP2 (gift from Johannes Loffing, ), CA9 (Invitrogen, PA1-16592), CD10 (Thermo Fisher Scientific PA5-47075), CD31 (Abcam, ab28364), pan-Cytokeratin (DAKO, M3515), HIF-1α (Novus Biotechnologies, NB-100-105), HIF-2α (PM8, gift from Patrick Pollard, ), NAPI2A (gift from Jürg Biber, ), NCC (Millipore, AB3553), PAX8 (Protein Tech Group, 10336-1-AP), Phospho-Thr202/Tyr204-Erk1/2 (Cell Signaling Technologies, 9101), Phospho-Thr37/46-4E-BP1 (Cell Signaling Technologies, 2855), pRB (BD Biosciences, 554136), THP (Santa Cruz Biotechnologies, SC-20631), acetylated-Tubulin (Sigma-Aldrich, T6793), vWF (Sigma, F3520). ..

    Article Title: Combined mutation of Vhl and Trp53 causes renal cysts and tumours in mice.
    Article Snippet: .. Antibodies, Western blotting, immunofluorescence and immunohistochemistry Western blotting, immunohistochemistry or immunofluorescence were conducted using previously described methods (Frew et al, 2008b) and the antibodies against the following epitopes: Acetylated tubulin (Sigma, #T6793), Actin (Sigma-Aldrich, A2228), AQP2 (Wagner et al, 2008), Aurora A (Abcam, ab13824), BubR1 (BD Biosciences, 612502), CDK-2 (Santa Cruz, sc-163-g), Cenp-E (Meraldi et al, 2004), E-cadherin (Abcam, ab11512), phospho-Thr37/46-4E-BP1 (Cell Signaling Technology, #2855), HIF1a (Novus Biologicals, NB100-105), HIF2a (Pollard et al, 2007, PM8), Ki67 (DakoCytomation, TEC-3), Mad2 (Bethyl Laboratories, A300301A), Myc (Epitomics, Y69), p53 (Novocastra, NCL-p53-CM5p), NaPi2 (Custer et al, 1994), NCC (Millipore, AB3553), phospho-Ser240/244-ribosomal S6 protein (Cell Signaling Technology, #2215), THP (Santa Cruz Biotechnology, sc-20631), pVHL(m)CT antibody (Hergovich et al, 2003), pVHL (Santa Cruz, sc-5575), Vimentin (Cell Signaling Technology, #5741). ..

    Immunofluorescence:

    Article Title: Combined Vhl , Trp53 and Rb1 mutation causes clear cell renal cell carcinoma in mice
    Article Snippet: .. Immunohistochemistry and immunofluorescence were conducted using previously described methods using the antibodies against the following antigens AQP1 (Abcam, ab15080), AQP2 (gift from Johannes Loffing, ), CA9 (Invitrogen, PA1-16592), CD10 (Thermo Fisher Scientific PA5-47075), CD31 (Abcam, ab28364), pan-Cytokeratin (DAKO, M3515), HIF-1α (Novus Biotechnologies, NB-100-105), HIF-2α (PM8, gift from Patrick Pollard, ), NAPI2A (gift from Jürg Biber, ), NCC (Millipore, AB3553), PAX8 (Protein Tech Group, 10336-1-AP), Phospho-Thr202/Tyr204-Erk1/2 (Cell Signaling Technologies, 9101), Phospho-Thr37/46-4E-BP1 (Cell Signaling Technologies, 2855), pRB (BD Biosciences, 554136), THP (Santa Cruz Biotechnologies, SC-20631), acetylated-Tubulin (Sigma-Aldrich, T6793), vWF (Sigma, F3520). ..

    Article Title: Combined mutation of Vhl and Trp53 causes renal cysts and tumours in mice.
    Article Snippet: .. Antibodies, Western blotting, immunofluorescence and immunohistochemistry Western blotting, immunohistochemistry or immunofluorescence were conducted using previously described methods (Frew et al, 2008b) and the antibodies against the following epitopes: Acetylated tubulin (Sigma, #T6793), Actin (Sigma-Aldrich, A2228), AQP2 (Wagner et al, 2008), Aurora A (Abcam, ab13824), BubR1 (BD Biosciences, 612502), CDK-2 (Santa Cruz, sc-163-g), Cenp-E (Meraldi et al, 2004), E-cadherin (Abcam, ab11512), phospho-Thr37/46-4E-BP1 (Cell Signaling Technology, #2855), HIF1a (Novus Biologicals, NB100-105), HIF2a (Pollard et al, 2007, PM8), Ki67 (DakoCytomation, TEC-3), Mad2 (Bethyl Laboratories, A300301A), Myc (Epitomics, Y69), p53 (Novocastra, NCL-p53-CM5p), NaPi2 (Custer et al, 1994), NCC (Millipore, AB3553), phospho-Ser240/244-ribosomal S6 protein (Cell Signaling Technology, #2215), THP (Santa Cruz Biotechnology, sc-20631), pVHL(m)CT antibody (Hergovich et al, 2003), pVHL (Santa Cruz, sc-5575), Vimentin (Cell Signaling Technology, #5741). ..

    Transgenic Assay:

    Article Title: Nutrient Sensing Nuclear Receptors Coordinate Autophagy
    Article Snippet: .. Reagents were obtained from the following sources: C57BL/6J mice and PPARα −/− mice from the Jackson Laboratory; FXR −/− and GFP-LC3 transgenic (Tg) mice were previously described , ; Antibodies to LC3 (NB600–1384) from Novus Biologicals; rabbit antibodies to phospho-Ser240/244 S6 (cat. # 5364), S6 (cat. # 2217), phospho-Thr37/46–4E–BP1 (cat. # 2855), 4E–BP1 (cat. # 9452), anti-mouse IgG-HRP (cat. # 7076) and β-actin (cat. # 5125) from Cell Signaling Technology; antibody to normal rabbit IgG (sc-2027), PPARα (sc-9000x), FXR (sc-13063x), Pol II (sc-899x), p300 (sc-585x), CRTC2/TORC2 (sc-6714x) and anti-rabbit IgG-HRP (sc-2370) from Santa Cruz Biotechnology; antibody to Histone H3K27me3 (cat. # 39155) from ACTIVE MOTIF; GW7647 and Wy-14,643 from Cayman chemicals; GW4064 from Tocris bioscience; Bafilomycin A 1 from Enzo Life Science; AML12 cells from ATCC (CRL-2254); GFP antibody (cat. # 11814460001), PhosSTOP (Cat. # 04906837001), and Complete Protease Cocktail (Cat. # 11836170001) from Roche; ITS (Cat. # 41400–045), Lipofectamine 2000 (Cat. # 11668019), BODIPY 493/503 (D-3922), Alexa Fluor 594 goat anti-rabbit IgG (Cat. # A11037), Stealth siRNAs-NCoR (NcoR1, MSS208758), SMRT (NcoR2, MSS20912), SHP (NR0b2, MSS239500), and HIGH_GC from Invitrogen; mRFP-GFP-LC3(ptfLC3) plasmid, a gift of T. Yoshimori (Addgene plasmid # 21074); anti-rabbit p62/SQSTM1 (Cat. # P0067), anti-rabbit LC3B (Cat. # L7543), Oleic-Acid-Albumin (Cat. # O3008–5ML), and Dexamethasone (Cat. # D4902–25MG) from Sigma; Protein A Sepharose CL-48 (Cat. # 17–0780–01) from GE Healthcare; VECTASHIELD (Cat. # H-1200) from Vector Laboratories; SacI-HF (Cat. # R3156S) and BglII (Cat. # R0144S) from New England Biolabs; PierceTM BCA Protein Assay Kit (Cat. # 23227) from Thermo Scientific; SMRT antibody (ab24551) and β-hydroxybutyrate assay kit (ab83390).from abcam; anti-acetyl-Histone H4 antibody (06–866) from EMD Millipore. ..

    Plasmid Preparation:

    Article Title: Nutrient Sensing Nuclear Receptors Coordinate Autophagy
    Article Snippet: .. Reagents were obtained from the following sources: C57BL/6J mice and PPARα −/− mice from the Jackson Laboratory; FXR −/− and GFP-LC3 transgenic (Tg) mice were previously described , ; Antibodies to LC3 (NB600–1384) from Novus Biologicals; rabbit antibodies to phospho-Ser240/244 S6 (cat. # 5364), S6 (cat. # 2217), phospho-Thr37/46–4E–BP1 (cat. # 2855), 4E–BP1 (cat. # 9452), anti-mouse IgG-HRP (cat. # 7076) and β-actin (cat. # 5125) from Cell Signaling Technology; antibody to normal rabbit IgG (sc-2027), PPARα (sc-9000x), FXR (sc-13063x), Pol II (sc-899x), p300 (sc-585x), CRTC2/TORC2 (sc-6714x) and anti-rabbit IgG-HRP (sc-2370) from Santa Cruz Biotechnology; antibody to Histone H3K27me3 (cat. # 39155) from ACTIVE MOTIF; GW7647 and Wy-14,643 from Cayman chemicals; GW4064 from Tocris bioscience; Bafilomycin A 1 from Enzo Life Science; AML12 cells from ATCC (CRL-2254); GFP antibody (cat. # 11814460001), PhosSTOP (Cat. # 04906837001), and Complete Protease Cocktail (Cat. # 11836170001) from Roche; ITS (Cat. # 41400–045), Lipofectamine 2000 (Cat. # 11668019), BODIPY 493/503 (D-3922), Alexa Fluor 594 goat anti-rabbit IgG (Cat. # A11037), Stealth siRNAs-NCoR (NcoR1, MSS208758), SMRT (NcoR2, MSS20912), SHP (NR0b2, MSS239500), and HIGH_GC from Invitrogen; mRFP-GFP-LC3(ptfLC3) plasmid, a gift of T. Yoshimori (Addgene plasmid # 21074); anti-rabbit p62/SQSTM1 (Cat. # P0067), anti-rabbit LC3B (Cat. # L7543), Oleic-Acid-Albumin (Cat. # O3008–5ML), and Dexamethasone (Cat. # D4902–25MG) from Sigma; Protein A Sepharose CL-48 (Cat. # 17–0780–01) from GE Healthcare; VECTASHIELD (Cat. # H-1200) from Vector Laboratories; SacI-HF (Cat. # R3156S) and BglII (Cat. # R0144S) from New England Biolabs; PierceTM BCA Protein Assay Kit (Cat. # 23227) from Thermo Scientific; SMRT antibody (ab24551) and β-hydroxybutyrate assay kit (ab83390).from abcam; anti-acetyl-Histone H4 antibody (06–866) from EMD Millipore. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Nutrient Sensing Nuclear Receptors Coordinate Autophagy
    Article Snippet: .. Reagents were obtained from the following sources: C57BL/6J mice and PPARα −/− mice from the Jackson Laboratory; FXR −/− and GFP-LC3 transgenic (Tg) mice were previously described , ; Antibodies to LC3 (NB600–1384) from Novus Biologicals; rabbit antibodies to phospho-Ser240/244 S6 (cat. # 5364), S6 (cat. # 2217), phospho-Thr37/46–4E–BP1 (cat. # 2855), 4E–BP1 (cat. # 9452), anti-mouse IgG-HRP (cat. # 7076) and β-actin (cat. # 5125) from Cell Signaling Technology; antibody to normal rabbit IgG (sc-2027), PPARα (sc-9000x), FXR (sc-13063x), Pol II (sc-899x), p300 (sc-585x), CRTC2/TORC2 (sc-6714x) and anti-rabbit IgG-HRP (sc-2370) from Santa Cruz Biotechnology; antibody to Histone H3K27me3 (cat. # 39155) from ACTIVE MOTIF; GW7647 and Wy-14,643 from Cayman chemicals; GW4064 from Tocris bioscience; Bafilomycin A 1 from Enzo Life Science; AML12 cells from ATCC (CRL-2254); GFP antibody (cat. # 11814460001), PhosSTOP (Cat. # 04906837001), and Complete Protease Cocktail (Cat. # 11836170001) from Roche; ITS (Cat. # 41400–045), Lipofectamine 2000 (Cat. # 11668019), BODIPY 493/503 (D-3922), Alexa Fluor 594 goat anti-rabbit IgG (Cat. # A11037), Stealth siRNAs-NCoR (NcoR1, MSS208758), SMRT (NcoR2, MSS20912), SHP (NR0b2, MSS239500), and HIGH_GC from Invitrogen; mRFP-GFP-LC3(ptfLC3) plasmid, a gift of T. Yoshimori (Addgene plasmid # 21074); anti-rabbit p62/SQSTM1 (Cat. # P0067), anti-rabbit LC3B (Cat. # L7543), Oleic-Acid-Albumin (Cat. # O3008–5ML), and Dexamethasone (Cat. # D4902–25MG) from Sigma; Protein A Sepharose CL-48 (Cat. # 17–0780–01) from GE Healthcare; VECTASHIELD (Cat. # H-1200) from Vector Laboratories; SacI-HF (Cat. # R3156S) and BglII (Cat. # R0144S) from New England Biolabs; PierceTM BCA Protein Assay Kit (Cat. # 23227) from Thermo Scientific; SMRT antibody (ab24551) and β-hydroxybutyrate assay kit (ab83390).from abcam; anti-acetyl-Histone H4 antibody (06–866) from EMD Millipore. ..

    Western Blot:

    Article Title: Combined mutation of Vhl and Trp53 causes renal cysts and tumours in mice.
    Article Snippet: .. Antibodies, Western blotting, immunofluorescence and immunohistochemistry Western blotting, immunohistochemistry or immunofluorescence were conducted using previously described methods (Frew et al, 2008b) and the antibodies against the following epitopes: Acetylated tubulin (Sigma, #T6793), Actin (Sigma-Aldrich, A2228), AQP2 (Wagner et al, 2008), Aurora A (Abcam, ab13824), BubR1 (BD Biosciences, 612502), CDK-2 (Santa Cruz, sc-163-g), Cenp-E (Meraldi et al, 2004), E-cadherin (Abcam, ab11512), phospho-Thr37/46-4E-BP1 (Cell Signaling Technology, #2855), HIF1a (Novus Biologicals, NB100-105), HIF2a (Pollard et al, 2007, PM8), Ki67 (DakoCytomation, TEC-3), Mad2 (Bethyl Laboratories, A300301A), Myc (Epitomics, Y69), p53 (Novocastra, NCL-p53-CM5p), NaPi2 (Custer et al, 1994), NCC (Millipore, AB3553), phospho-Ser240/244-ribosomal S6 protein (Cell Signaling Technology, #2215), THP (Santa Cruz Biotechnology, sc-20631), pVHL(m)CT antibody (Hergovich et al, 2003), pVHL (Santa Cruz, sc-5575), Vimentin (Cell Signaling Technology, #5741). ..



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